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- Make it stand out Avoid tragedy https://www.alevelbiologytutor.com/tutoring-blog/2024/3/12/exam-tips-for-a-level-biology-graph-questions monthly 0.5 2025-04-02 https://images.squarespace-cdn.com/content/v1/56d845f922482efd8c173d16/b78b41d8-a717-4305-bfde-9a77fb8a873e/graphquestion.jpg Biology A level Answers - How to Approach A level Biology Graph and Table Questions: Tips and Exam Question Pack - Make it stand out Don’t panic, it’s only a graph https://images.squarespace-cdn.com/content/v1/56d845f922482efd8c173d16/f982bf30-49a0-4709-a2de-e5eff50ef8ab/table.jpg Biology A level Answers - How to Approach A level Biology Graph and Table Questions: Tips and Exam Question Pack - Make it stand out What’s going on here? https://images.squarespace-cdn.com/content/v1/56d845f922482efd8c173d16/c0b1badc-3cfe-4923-8bb6-8ed25b912662/image-asset.png Biology A level Answers - How to Approach A level Biology Graph and Table Questions: Tips and Exam Question Pack - Make it stand out Whatever it is, the way you tell your story online can make all the difference. https://www.alevelbiologytutor.com/tutoring-blog/2024/3/1/transport-in-animals-understanding-haemoglobin-cooperative-binding-and-oxygen-dissociation-curves monthly 0.5 2025-03-13 https://images.squarespace-cdn.com/content/v1/56d845f922482efd8c173d16/e06c53c8-965f-454c-a8f2-3272a822715c/Blood.jpg Biology A level Answers - Transport in Animals: Haemoglobin, Oxygen Dissociation Curves, and the Bohr Effect - Make it stand out Red blood cells stuffed full of haemoglobin. Their bright red colour tells us their haemoglobin is in the form of oxyhaemoglobin, with bound oxygen. https://images.squarespace-cdn.com/content/v1/56d845f922482efd8c173d16/7fa5ca87-6647-4b5b-9490-faa260df601b/Everest.jpg Biology A level Answers - Transport in Animals: Haemoglobin, Oxygen Dissociation Curves, and the Bohr Effect - Make it stand out The percentage of Oxygen in the air is the same on Everest as at sea level. https://images.squarespace-cdn.com/content/v1/56d845f922482efd8c173d16/5a80333b-f4d9-4752-8396-dc7d72b13533/Haemoglobin.png Biology A level Answers - Transport in Animals: Haemoglobin, Oxygen Dissociation Curves, and the Bohr Effect - Make it stand out Haemoglobin - there are four polypeptide chains, here two are shown in green, and two in red (apologies to anyone who is colour-blind, I didn’t make this graphic). Each polypeptide chain is folded to create a subunit of the protein, and each subunit provides a binding site which can bind one Oxygen molecule. https://images.squarespace-cdn.com/content/v1/56d845f922482efd8c173d16/677f8a10-7c50-44f1-b445-a5282b070759/OxyDissoc.jpg Biology A level Answers - Transport in Animals: Haemoglobin, Oxygen Dissociation Curves, and the Bohr Effect - Make it stand out The much-feared oxygen dissociation curve. The really interesting thing is that it is not a straight line. You need to know why not, and why this is crucial for oxygen delivery to the body tissues. https://images.squarespace-cdn.com/content/v1/56d845f922482efd8c173d16/592ffbdf-f900-471a-9caf-e1a3d33e8e83/OxyDissocCO2.jpg Biology A level Answers - Transport in Animals: Haemoglobin, Oxygen Dissociation Curves, and the Bohr Effect - Make it stand out Notice that the right-shifted, red curve is lower than the blue one. This tells us that at high CO2, haemoglobin has LESS affinity for oxygen. (The colours don’t mean anything.) https://www.alevelbiologytutor.com/tutoring-blog/2024/3/1/exploring-biological-molecules-understanding-amino-acids-protein-structure-and-function monthly 0.5 2026-01-27 https://images.squarespace-cdn.com/content/v1/56d845f922482efd8c173d16/f18d2541-3daf-408c-a91a-c54452d188b8/Coronavirus_Spike_Protein.jpg Biology A level Answers - Exploring Biological Molecules: Amino Acids, Protein Structure, and Function - Make it stand out Coronavirus Spike Protein https://images.squarespace-cdn.com/content/v1/56d845f922482efd8c173d16/1709722780581-M9VQYNKRY4DENEGY0CDG/308px-L-Asparagine_zwitterion_ball_from_xtal.png Biology A level Answers - Exploring Biological Molecules: Amino Acids, Protein Structure, and Function - Make it stand out The amino acid Asparagine. The amino group is shown sticking up at the top. The carboxyl group is to the right, and the R group (CH2-CO-NH2) is sticking out to the left. https://images.squarespace-cdn.com/content/v1/56d845f922482efd8c173d16/c97689b3-0bd3-4c17-9b03-88c251e0962c/lipocaine_publicdomain.jpg Biology A level Answers - Exploring Biological Molecules: Amino Acids, Protein Structure, and Function - Make it stand out Lipocaine protein. Its secondary structures of alpha helix (yellow) and beta sheet (blue) are packed together to form a tertiary structure. https://www.alevelbiologytutor.com/tutoring-blog/2023/12/4/how-to-revise-a-level-biology-learn-the-language monthly 0.5 2025-03-22 https://images.squarespace-cdn.com/content/v1/56d845f922482efd8c173d16/61e3a300-0a95-4048-8ca9-783b2d1dee35/letters.jpg Biology A level Answers - How to Revise A Level Biology: Learn the Language - Make it stand out Whatever it is, the way you tell your story online can make all the difference. https://www.alevelbiologytutor.com/tutoring-blog/2024/2/29/key-concept-polar-vs-charged monthly 0.5 2025-10-10 https://images.squarespace-cdn.com/content/v1/56d845f922482efd8c173d16/d1104f93-f027-4823-a1af-e92d78eb4b65/5+polar+delta.png Biology A level Answers - Key Concept: Polar and Charged Molecules - Make it stand out This molecule is polar, but not charged. All is explained below. https://images.squarespace-cdn.com/content/v1/56d845f922482efd8c173d16/7547ffd2-8abe-4778-8a59-a6ce94743e7b/1+Atom.png Biology A level Answers - Key Concept: Polar and Charged Molecules - Atom The atoms that make up molecules each have a postively-charged nucleus and a cloud of negatively-charged electrons. Different types of atoms have different numbers of charges. This means that even non-charged, non-polar molecules contain charges! They just cancel each other out so you don’t notice them. https://images.squarespace-cdn.com/content/v1/56d845f922482efd8c173d16/71b30ece-7b36-4075-8b53-da4a1343e86b/2+Nonpolar.png Biology A level Answers - Key Concept: Polar and Charged Molecules - Non-polar When you make a molecule out of atoms, electrons are shared between neighbouring atoms. The electrons become one big shared cloud. This makes a covalent bond. The diagram shows a non-polar molecule with two atoms. In a non-polar molecule, all the charges are balanced, cancelling each other out. Because the charges are distributed evenly, and cancel out overall, the molecule behaves as though there are no charges (in terms of its electrostatic interactions with other nearby molecules). So why are they called “non-polar”? To understand that, you need to understand what polar means. https://images.squarespace-cdn.com/content/v1/56d845f922482efd8c173d16/433bbbf3-e732-4b29-99eb-26ee089aa014/3+polar.png Biology A level Answers - Key Concept: Polar and Charged Molecules - Polar It turns out that some types of atomic nuclei just LOVE electrons. Like, they are particularly greedy for them. Oxygen, for example. These greedy atoms yank the electron cloud over towards their nucleus, away from the nucleus of the other atom. https://images.squarespace-cdn.com/content/v1/56d845f922482efd8c173d16/0e8eb34f-8a7a-44a1-98b7-87db156c4e46/little+bit+more.png Biology A level Answers - Key Concept: Polar and Charged Molecules The other atom no longer has enough negative charge to cancel out its positively-charged nucleus. While the greedy one has more negatively charged electrons than it needs. The charges no longer cancel each other out. The other atom now has just a little bit of a positive charge, and the greedy one has just a little bit of a negative charge. https://images.squarespace-cdn.com/content/v1/56d845f922482efd8c173d16/d1104f93-f027-4823-a1af-e92d78eb4b65/5+polar+delta.png Biology A level Answers - Key Concept: Polar and Charged Molecules This is a polar molecule. Its atoms still share one electron cloud, so they are still covalently bonded. But the small charge in charge distribution mean it will now interact differently with its environment. Overall, the charges still cancel out. They are just unbalanced so that there are places with just a little bit of charge. Saying "just a little bit” of charge is a pain, so instead the delta symbol is used to show this. δ+ = just a little bit of positive charge δ- = just a little bit of negative charge https://images.squarespace-cdn.com/content/v1/56d845f922482efd8c173d16/df8bdfb9-f824-4c9f-81f5-36dbbc68367d/Hydroxyl_Glucose.jpg Biology A level Answers - Key Concept: Polar and Charged Molecules This can also happen to just one part of a molecule. A good example is a hydroxyl group (OH). The oxygen pulls the electrons toward it, so that there is just a little bit (δ) of charge on the oxygen and hydrogen atoms. Molecules with hydroxyl groups are polar. Look at glucose - it has loads of hydroxyl groups; this is what makes it a polar molecule. This is important for how it behaves in water, but before we get to that, let’s look at how charged atoms/molecules are different: https://images.squarespace-cdn.com/content/v1/56d845f922482efd8c173d16/d1104f93-f027-4823-a1af-e92d78eb4b65/5+polar+delta.png Biology A level Answers - Key Concept: Polar and Charged Molecules - Polar A polar molecule has no overall charge. The charge of its positive nuclei exactly cancel out the charge of its negative electrons. The charges are just unevenly distributed, giving a little bit (δ) of positive charge to one atom, and slight negative charge to another atom. In biology, you’ll normally find it’s a hydrogen atom that has had its electrons yanked away and is now carrying a little bit of positive charge. https://images.squarespace-cdn.com/content/v1/56d845f922482efd8c173d16/071681d7-14e0-47af-8465-72921b6b5c53/6+no+covalent.png Biology A level Answers - Key Concept: Polar and Charged Molecules - Charged Now look at this. These atoms are not sharing a cloud of electrons - the big one has gone all-in and taken the whole lot for itself. No shared electron cloud means there is no covalent bond. No covalent bond means they are no longer a single molecule, but rather two separate atoms … well, except that they’re not even atoms any more … https://images.squarespace-cdn.com/content/v1/56d845f922482efd8c173d16/919654d6-c739-4a25-91d5-a07953d816a1/7+full+charged.png Biology A level Answers - Key Concept: Polar and Charged Molecules The atoms no longer just have just a little bit (δ) of charge. The one on the left has lost an entire electron’s worth of charge. Losing negative charge means that overall it is now (properly, not just a little bit) positively charged. The one on the right has a whole electron’s worth of negative charge more than it needs to cancel out its positive nuclues. It is now (properly) negatively charged. Because they are (properly!) charged, we no longer call them atoms. Instead they are ions. https://images.squarespace-cdn.com/content/v1/56d845f922482efd8c173d16/38f4cc5b-98c8-48e1-bf2f-20925dba074d/12+water+dissociated.png Biology A level Answers - Key Concept: Polar and Charged Molecules Water experiences this sort of electron-theft. Sometimes it exists as the polar H20 molecule, but sometimes its oxygen gets even more greedy and the molecule dissociates into H+ (a hydrogen ion, aka proton), and OH- (a hydroxyl ion). This dissociation, and the reforming of H20, is happening all the time in normal liquid water. Note that the ions each have an overall charge, unlike the polar water molecule where the small charges cancel out. https://images.squarespace-cdn.com/content/v1/56d845f922482efd8c173d16/012c05cd-6b3c-46c3-b7cc-4b102211e4d6/9+amino.png Biology A level Answers - Key Concept: Polar and Charged Molecules Here’s an amine group. It’s just part of a larger molecule, which goes off the edge of the image. It can exist either as —NH2, or it can add on a proton (H+) to become —NH3+. In living organisms, there are plenty of available protons (remember how water dissociates?). So amine groups like this usually exist as the charged version. This is not a polar group, it is charged. (Ignore the shape of the electron cloud for this one, the important thing is that there is an overall charge of +1 because of that extra proton). https://images.squarespace-cdn.com/content/v1/56d845f922482efd8c173d16/1987a840-db8f-4edb-baa4-a294f1b688ce/10+h+bonds.png Biology A level Answers - Key Concept: Polar and Charged Molecules - Hydrogen bonds Some polar molecules, like DNA, proteins and water, can form hydrogen bonds between the atoms that have the unevenly distributed charges. These are a special type of weak bond. Water LOVES making hydrogen bonds - this is why it can hold itself together into a droplet. Notice in the picture that the water molecules remain separate and can still move around. It doesn’t take much to pull a single hydrogen bond apart. Which is why water can still be poured and stirred around with no trouble. Water is a polar, hydrogen-bonding molecule, and this explains its properties as a solvent. Molecules like glucose can dissolve in water because they are similarly polar and able to make hydrogen bonds. https://images.squarespace-cdn.com/content/v1/56d845f922482efd8c173d16/b5fd6868-ccf5-4aa0-a09a-ec70a94e5bf8/DNA.jpg Biology A level Answers - Key Concept: Polar and Charged Molecules Hydrogen bonds are also really important in understanding DNA and protein structures. These molecules hydrogen-bond to themselves. Each individual bond is weak, but multiple repeating bonds work together to hold the structure into shape. Protein secondary structures are held together by hydrogen bonds. The image here shows hydrogen bonds between Guanine (G) and Cytosine (C) in DNA. The hydrogen bonds are shown as dotted lines. https://images.squarespace-cdn.com/content/v1/56d845f922482efd8c173d16/93ea61d6-7520-458d-b38f-f20c14b763c4/13+Na+Cl+in+water.png Biology A level Answers - Key Concept: Polar and Charged Molecules - Dissolving ions Charged ions like Cl-, Na+ and K+ can’t form hydrogen bonds, but they can still dissolve in water because they can form favourable electrostatic interactions with the water molecules. This diagram shows salt (NaCl) dissolved in water. Hydrogen bonds are in yellow. And electrostatic interactions between the charged ions and the polar water molecules are shown in green. See how the water molecules organise around the ions to provide the opposite charge to that presented by the ion. https://images.squarespace-cdn.com/content/v1/56d845f922482efd8c173d16/55f58a40-5e90-4592-8dfa-cb625f8cd0fe/11+salt+bridge.png Biology A level Answers - Key Concept: Polar and Charged Molecules - Ionic bonds Charged molecules have ‘proper’ charges. They interact more strongly through electrostatic interactions to form ionic bonds. Here is a positively charged amine group (NH3+) forming an ionic bond with a negatively charged hydroxyl group (OH-). They are not sharing an electron cloud, so this is not a covalent bond. Maybe these charged groups are both parts of the same protein (ie from different R groups). If so, this interaction may be important in defining the protein’s tertiary structure. Or maybe it’s an interaction between an enzyme and its substrate? Ionic bonds are really important for controlling what binds with what - and what doesn’t. Negatively charged groups will repel other negative charges. And positive will repel positive. 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